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Primary Antibodies Against Sars Cov 2 Spike Protein Gtx632604, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pathological analysis of the lungs of MA‐P10‐infected‐mice. (a) (Left) Representative images of the mice lungs stained with H&E. Scale bars, top: 300 μm, bottom: 100 μm (Right). The rates of inflammatory areas in the lungs of infected young and mid‐aged mice at 4 dpi were quantified. Statistical analysis was performed using a Mann–Whitney test, where ** p < 0.01 (infected young: n = 6; infected mid‐aged: n = 6). (b) Representative immunohistochemistry images of SARS‐CoV‐2 nucleocapsid protein (N protein) in the lungs of infected mice (left, Scale bar, 100 μm). The rate of N protein positive areas in the whole mice lungs and alveoli were measured (right). Statistical analysis was performed using a Mann–Whitney test, where **** p < 0.0001 (infected young: n = 9; infected mid‐aged: n = 9). (c) CD68 and (d) MPO in the mice lungs (left, Scale bar, 100 μm) and positive areas in the whole mice lungs or N protein positive area (right). Statistical analysis was carried out using a Mann–Whitney test, where * p < 0.05; ns, not significant. (e) Representative immunohistochemistry images of CD41 in the lungs of infected mice (left, Scale bar, 100 μm). The CD41‐positive areas in the whole mice lungs (middle) or in the N protein positive areas were measured (right). Statistical analysis was performed using a Mann–Whitney test. ns, not significant. (f) Rate of blood vessels including CD41 positive signals. Statistical analysis was accomplished using a Mann–Whitney test, where **** p < 0.0001 (Infected young: n = 9; infected mid‐aged: n = 9). (g) The representative serial section images of CD31 and N protein staining (middle, scale bar, 25 μm). (h) The representative immunofluorescence image of alveolar capillary vessels of CD31 and <t>S</t> <t>protein</t> staining (scale bar, 10 μm).
Primary Antibodies Against Sars‑Cov‑2 Spike Protein Gtx632604, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pathological analysis of the lungs of MA‐P10‐infected‐mice. (a) (Left) Representative images of the mice lungs stained with H&E. Scale bars, top: 300 μm, bottom: 100 μm (Right). The rates of inflammatory areas in the lungs of infected young and mid‐aged mice at 4 dpi were quantified. Statistical analysis was performed using a Mann–Whitney test, where ** p < 0.01 (infected young: n = 6; infected mid‐aged: n = 6). (b) Representative immunohistochemistry images of SARS‐CoV‐2 nucleocapsid protein (N protein) in the lungs of infected mice (left, Scale bar, 100 μm). The rate of N protein positive areas in the whole mice lungs and alveoli were measured (right). Statistical analysis was performed using a Mann–Whitney test, where **** p < 0.0001 (infected young: n = 9; infected mid‐aged: n = 9). (c) CD68 and (d) MPO in the mice lungs (left, Scale bar, 100 μm) and positive areas in the whole mice lungs or N protein positive area (right). Statistical analysis was carried out using a Mann–Whitney test, where * p < 0.05; ns, not significant. (e) Representative immunohistochemistry images of CD41 in the lungs of infected mice (left, Scale bar, 100 μm). The CD41‐positive areas in the whole mice lungs (middle) or in the N protein positive areas were measured (right). Statistical analysis was performed using a Mann–Whitney test. ns, not significant. (f) Rate of blood vessels including CD41 positive signals. Statistical analysis was accomplished using a Mann–Whitney test, where **** p < 0.0001 (Infected young: n = 9; infected mid‐aged: n = 9). (g) The representative serial section images of CD31 and N protein staining (middle, scale bar, 25 μm). (h) The representative immunofluorescence image of alveolar capillary vessels of CD31 and <t>S</t> <t>protein</t> staining (scale bar, 10 μm).
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Pathological analysis of the lungs of MA‐P10‐infected‐mice. (a) (Left) Representative images of the mice lungs stained with H&E. Scale bars, top: 300 μm, bottom: 100 μm (Right). The rates of inflammatory areas in the lungs of infected young and mid‐aged mice at 4 dpi were quantified. Statistical analysis was performed using a Mann–Whitney test, where ** p < 0.01 (infected young: n = 6; infected mid‐aged: n = 6). (b) Representative immunohistochemistry images of SARS‐CoV‐2 nucleocapsid protein (N protein) in the lungs of infected mice (left, Scale bar, 100 μm). The rate of N protein positive areas in the whole mice lungs and alveoli were measured (right). Statistical analysis was performed using a Mann–Whitney test, where **** p < 0.0001 (infected young: n = 9; infected mid‐aged: n = 9). (c) CD68 and (d) MPO in the mice lungs (left, Scale bar, 100 μm) and positive areas in the whole mice lungs or N protein positive area (right). Statistical analysis was carried out using a Mann–Whitney test, where * p < 0.05; ns, not significant. (e) Representative immunohistochemistry images of CD41 in the lungs of infected mice (left, Scale bar, 100 μm). The CD41‐positive areas in the whole mice lungs (middle) or in the N protein positive areas were measured (right). Statistical analysis was performed using a Mann–Whitney test. ns, not significant. (f) Rate of blood vessels including CD41 positive signals. Statistical analysis was accomplished using a Mann–Whitney test, where **** p < 0.0001 (Infected young: n = 9; infected mid‐aged: n = 9). (g) The representative serial section images of CD31 and N protein staining (middle, scale bar, 25 μm). (h) The representative immunofluorescence image of alveolar capillary vessels of CD31 and <t>S</t> <t>protein</t> staining (scale bar, 10 μm).
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Pathological analysis of the lungs of MA‐P10‐infected‐mice. (a) (Left) Representative images of the mice lungs stained with H&E. Scale bars, top: 300 μm, bottom: 100 μm (Right). The rates of inflammatory areas in the lungs of infected young and mid‐aged mice at 4 dpi were quantified. Statistical analysis was performed using a Mann–Whitney test, where ** p < 0.01 (infected young: n = 6; infected mid‐aged: n = 6). (b) Representative immunohistochemistry images of SARS‐CoV‐2 nucleocapsid protein (N protein) in the lungs of infected mice (left, Scale bar, 100 μm). The rate of N protein positive areas in the whole mice lungs and alveoli were measured (right). Statistical analysis was performed using a Mann–Whitney test, where **** p < 0.0001 (infected young: n = 9; infected mid‐aged: n = 9). (c) CD68 and (d) MPO in the mice lungs (left, Scale bar, 100 μm) and positive areas in the whole mice lungs or N protein positive area (right). Statistical analysis was carried out using a Mann–Whitney test, where * p < 0.05; ns, not significant. (e) Representative immunohistochemistry images of CD41 in the lungs of infected mice (left, Scale bar, 100 μm). The CD41‐positive areas in the whole mice lungs (middle) or in the N protein positive areas were measured (right). Statistical analysis was performed using a Mann–Whitney test. ns, not significant. (f) Rate of blood vessels including CD41 positive signals. Statistical analysis was accomplished using a Mann–Whitney test, where **** p < 0.0001 (Infected young: n = 9; infected mid‐aged: n = 9). (g) The representative serial section images of CD31 and N protein staining (middle, scale bar, 25 μm). (h) The representative immunofluorescence image of alveolar capillary vessels of CD31 and S protein staining (scale bar, 10 μm).

Journal: Aging Cell

Article Title: Viral uptake and pathophysiology of the lung endothelial cells in age‐associated severe SARS‐CoV ‐2 infection models

doi: 10.1111/acel.14050

Figure Lengend Snippet: Pathological analysis of the lungs of MA‐P10‐infected‐mice. (a) (Left) Representative images of the mice lungs stained with H&E. Scale bars, top: 300 μm, bottom: 100 μm (Right). The rates of inflammatory areas in the lungs of infected young and mid‐aged mice at 4 dpi were quantified. Statistical analysis was performed using a Mann–Whitney test, where ** p < 0.01 (infected young: n = 6; infected mid‐aged: n = 6). (b) Representative immunohistochemistry images of SARS‐CoV‐2 nucleocapsid protein (N protein) in the lungs of infected mice (left, Scale bar, 100 μm). The rate of N protein positive areas in the whole mice lungs and alveoli were measured (right). Statistical analysis was performed using a Mann–Whitney test, where **** p < 0.0001 (infected young: n = 9; infected mid‐aged: n = 9). (c) CD68 and (d) MPO in the mice lungs (left, Scale bar, 100 μm) and positive areas in the whole mice lungs or N protein positive area (right). Statistical analysis was carried out using a Mann–Whitney test, where * p < 0.05; ns, not significant. (e) Representative immunohistochemistry images of CD41 in the lungs of infected mice (left, Scale bar, 100 μm). The CD41‐positive areas in the whole mice lungs (middle) or in the N protein positive areas were measured (right). Statistical analysis was performed using a Mann–Whitney test. ns, not significant. (f) Rate of blood vessels including CD41 positive signals. Statistical analysis was accomplished using a Mann–Whitney test, where **** p < 0.0001 (Infected young: n = 9; infected mid‐aged: n = 9). (g) The representative serial section images of CD31 and N protein staining (middle, scale bar, 25 μm). (h) The representative immunofluorescence image of alveolar capillary vessels of CD31 and S protein staining (scale bar, 10 μm).

Article Snippet: An immunohistochemistry assay was employed using several primary antibodies against SARS‐CoV‐2 spike protein (mouse, GTX632604, GeneTex), SARS‐CoV‐2 nucleocapsid protein (rabbit, GTX635679, GeneTex), CD31 (rabbit, ab28364, Abcam), CD41 (rabbit, ab134131, Abcam), CD45 (rat, #103202, Biolegend), MPO (rabbit, ab9535, Abcam), CD68 (rabbit, ab125212, Abcam), and P‐selectin/CD62P (rabbit, ab255822, Abcam).

Techniques: Infection, Staining, MANN-WHITNEY, Immunohistochemistry, Immunofluorescence

Pathological analysis of the lungs of MA‐P10‐infected‐mice. (a) (Left) Representative images of the mice lungs stained with H&E. Scale bars, top: 300 μm, bottom: 100 μm (Right). The rates of inflammatory areas in the lungs of infected young and mid‐aged mice at 4 dpi were quantified. Statistical analysis was performed using a Mann–Whitney test, where ** p < 0.01 (infected young: n = 6; infected mid‐aged: n = 6). (b) Representative immunohistochemistry images of SARS‐CoV‐2 nucleocapsid protein (N protein) in the lungs of infected mice (left, Scale bar, 100 μm). The rate of N protein positive areas in the whole mice lungs and alveoli were measured (right). Statistical analysis was performed using a Mann–Whitney test, where **** p < 0.0001 (infected young: n = 9; infected mid‐aged: n = 9). (c) CD68 and (d) MPO in the mice lungs (left, Scale bar, 100 μm) and positive areas in the whole mice lungs or N protein positive area (right). Statistical analysis was carried out using a Mann–Whitney test, where * p < 0.05; ns, not significant. (e) Representative immunohistochemistry images of CD41 in the lungs of infected mice (left, Scale bar, 100 μm). The CD41‐positive areas in the whole mice lungs (middle) or in the N protein positive areas were measured (right). Statistical analysis was performed using a Mann–Whitney test. ns, not significant. (f) Rate of blood vessels including CD41 positive signals. Statistical analysis was accomplished using a Mann–Whitney test, where **** p < 0.0001 (Infected young: n = 9; infected mid‐aged: n = 9). (g) The representative serial section images of CD31 and N protein staining (middle, scale bar, 25 μm). (h) The representative immunofluorescence image of alveolar capillary vessels of CD31 and S protein staining (scale bar, 10 μm).

Journal: Aging Cell

Article Title: Viral uptake and pathophysiology of the lung endothelial cells in age‐associated severe SARS‐CoV ‐2 infection models

doi: 10.1111/acel.14050

Figure Lengend Snippet: Pathological analysis of the lungs of MA‐P10‐infected‐mice. (a) (Left) Representative images of the mice lungs stained with H&E. Scale bars, top: 300 μm, bottom: 100 μm (Right). The rates of inflammatory areas in the lungs of infected young and mid‐aged mice at 4 dpi were quantified. Statistical analysis was performed using a Mann–Whitney test, where ** p < 0.01 (infected young: n = 6; infected mid‐aged: n = 6). (b) Representative immunohistochemistry images of SARS‐CoV‐2 nucleocapsid protein (N protein) in the lungs of infected mice (left, Scale bar, 100 μm). The rate of N protein positive areas in the whole mice lungs and alveoli were measured (right). Statistical analysis was performed using a Mann–Whitney test, where **** p < 0.0001 (infected young: n = 9; infected mid‐aged: n = 9). (c) CD68 and (d) MPO in the mice lungs (left, Scale bar, 100 μm) and positive areas in the whole mice lungs or N protein positive area (right). Statistical analysis was carried out using a Mann–Whitney test, where * p < 0.05; ns, not significant. (e) Representative immunohistochemistry images of CD41 in the lungs of infected mice (left, Scale bar, 100 μm). The CD41‐positive areas in the whole mice lungs (middle) or in the N protein positive areas were measured (right). Statistical analysis was performed using a Mann–Whitney test. ns, not significant. (f) Rate of blood vessels including CD41 positive signals. Statistical analysis was accomplished using a Mann–Whitney test, where **** p < 0.0001 (Infected young: n = 9; infected mid‐aged: n = 9). (g) The representative serial section images of CD31 and N protein staining (middle, scale bar, 25 μm). (h) The representative immunofluorescence image of alveolar capillary vessels of CD31 and S protein staining (scale bar, 10 μm).

Article Snippet: After PBS wash, sections were incubated with 5% Goat serum in 1 × PBS for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against SARS‐CoV‐2 spike protein (mouse, GTX632604, GeneTex) and CD31 (rabbit, ab28364, Abcam) in PBS containing 5% goat serum.

Techniques: Infection, Staining, MANN-WHITNEY, Immunohistochemistry, Immunofluorescence